desmoglein 1 antibody

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. Desmoglein Antibodies (1 and 3) Test Resources. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Please Note: Optimal dilutions should be determined by each laboratory for each application. Desmoglein-1 is a member of the desmoglein protein subfamily. pemphigus and differential diagnosis of pemphigoid, EBA etc.MethodELISA with recombinant antigens desmoglein 1 and 3. [email protected] No anticoagulants, or preservatives should be added. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Enzyme Linked Immunosorbent Assay (ELISA) Methodology. These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. Desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g. 12 months from date of receipt, -20 to -70 °C as supplied. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. Setting: Dermatology departments from 13 university hospitals in France. Desmoglein 1 is a desmosomal cadherin with a molecular weight of 160kD, whereas desmoglein 3 has a molecular weight of 130kD. Bio-Techne Tissue-tek Expand Tissue-tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR. Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. These desmoglein gene family members are located in a cluster on chromosome 18. are available in the Technical Information section on our website. Reconstitute at 0.5 mg/mL in sterile PBS. 2001 Apr;144(4):775-80. Harman KE(1), Seed PT, Gratian MJ, Bhogal BS, Challacombe SJ, Black MM. Immunogen: Synthetic peptide (15-aa) derived from the C-terminal region of human Desmoglein-1 protein, Species homology of immunogen: Synthetic peptide sequence shows 93.3% homology to mouse or rat sequence, Storage buffer: PBS, pH 7.2, 0.1% sodium azide. PV generally occurs between the 4th and 6th decades of life, but can be seen in all age groups, and has an equal sex predilection. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Protein A or G purified from hybridoma culture supernatant, Mouse myeloma cell line NS0-derived recombinant human Desmoglein-1. Specific staining was localized to keratinocyte membranes. Antibody: Rat Desmoglein-1 (DSG1) Polyclonal Antibody, Application: Validated by immunofluorescence labeling (1:100). Recombinant Human Desmoglein‑1 Fc Chimera (Catalog #. Upon receipt, store it immediately at -20 to -70 °C, Cadherin family member 4; CDHF4; CDHF4PPKS1; desmoglein 1; Desmoglein1; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DGI; DSG; DSG1; Pemphigus foliaceus antigen; SPPK1. Design: Retrospective study. The product is shipped at ambient temperature. Desmoglein-1 (DSG1) antibody labeling of the developing skin. Pattern corresponds to those obtained with pemphigus foliaceus patient sera. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. (Based on 2 Reviews), $25/€18/£15/$25CAN/Â¥75 Yuan/Â¥1250 Yen for a review with an image, $10/€7/£6/$10 CAD/Â¥70 Yuan/Â¥1110 Yen for a review without an image. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Consider ordering with Epithelial Cell Surface Antibody IgG . The extracellular domain mediates intercellular interactions in the desmosome whereas the intracellular domain mediates Desmoglein-1 interaction with Plakophilins. Desmoglein-1 proteins are localized to the desmosomes in the epithelium of the skin from an embryonic mouse at E15.5. WHERE SCIENCE INTERSECTS INNOVATIONTM. Blood should be collected fasting or at least one hour after meals to avoid li… Citations: Showing 1 - 2 VAT, excl. Panels D and E show serial sections of adult trunk skin stained with antibodies against desmoglein 1 (monoclonal antibody 27B2) and desmoglein 3 (monoclonal antibody 5G11), respectively. These desmoglein gene family members are located in a cluster on chromosome 18. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well. To better understand PF pathophysiology, we used phage … Cat. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters. Materials and reagents Expand Equipment Leica CM1950 automatic sectioning cryostat.Thermo Fisher Scientific round (12 mm diameter) cover glass (thickness No 1.5).Thermo Fisher Scientific ColorFrostTM Plus slides made of positively charge uncoated glass. Desmoglein-1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Antibody titer correlates in a semiquantitative manner with … Citations for Human Desmoglein-1 Antibody, Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services, IHC Staining with VisUCyte HRP Polymer Detection Reagents, Streptococcal Cysteine Protease-Mediated Cleavage of Desmogleins Is Involved in the Pathogenesis of Cutaneous Infection. Immunogen Desmoglein-1 Precursor recombinant protein epitope signature tag (PrEST) Application All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org) and as a result, are supported by the most extensive characterization in the industry. Marke MaxPab® Kreuzreaktivität Human Immunogen. Desmoglein‑1 in Human Skin Hyperplasia. Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg Secondary HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 114 kDa. products description : Mouse monoclonal Desmoglein 1 antibody. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Circulating IgG antibodies to desmoglein-1 and -3 (Dsg1, Dsg3) adhesion molecules of squamous epithelial cells are the underlying defects, causing loss of cell-to-cell adhesion. Pemphigus vulgaris patients with both mucosal and skin lesions have antibodies to both DSG-3 and DSG-1. Desmoglein-1 is encoded by the DSG1 gene in human. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. ncbi gi num : 119703744. ncbi acc num : NP_001933.2. Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. 6 months, -20 to -70 °C under sterile conditions after reconstitution. Excl. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. The desmoglein-1 subtype is highly expressed in the epidermis. Do not use heat-inactivated serum as this affects test results. but also provides information about sample types, species, and experimental conditions. A direct immunofluorescence test showed intercellular space deposits of IgG, and the ELISA index to anti-desmoglein 1 antibodies was raised to 115 (reference range <20). For Research Use Only. Buffers Expand BLOCK Solution  NaCl 8 gKCl 0.2 gNa2HPO4 1.44 gKH2PO4 […], Antibody: Rabbit VE-cadherin (Cdh5 or Cadherin-5 or CD144) Polyclonal Antibody, Antibody: Rabbit Anti-Claudin-10 (CLDN10) Polyclonal Antibody, Antibody: Rabbit Anti-ZO-1 (Tjp1) (N-terminus) polyclonal antibody. Methodology. $290.00. Aseptically collect 5-8 mL of blood by approved venipuncture procedures. None found for this test Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities. Test Details. Br J Dermatol. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Customer Support: [email protected], Technical Issues: [email protected], Validated Antibodies – The Foundation of Biomedical Research. The protein encoded by this gene has been identified as the autoantigen of the autoim… Desmoglein versus non-desmoglein signaling in pemphigus acantholysis: characterization of novel signaling pathways downstream of pemphigus vulgaris antigens. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Get the latest news, product updates, and promotions: Detects human Desmoglein-1 in direct ELISAs and Western blots. Reconstructed human epidermis tissues were embedded in paraffin. © Copyright 2021 R&D Systems, Inc. All Rights Reserved. The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels. General Protocols These antibodies bind to calcium dependent adhesion molecules in cell surface desmosomes, notably desmoglein 1(DSG-1) in pemphigus foliaceus and desmoglein 3 (DSG-3) in pemphigus vulgaris. ncbi gb acc num : NM_001942.2. 2 The monoclonal anti-desmoglein 1 antibody stains basal and lower suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Desmoglein-1 Antigen Affinity-purified Polyclonal Antibody (Catalog … Paraffin was removed by successive baths of xylene and ethanol; then, antigen was retrieved using citrate. The data collected includes not only links to publications in PubMed, of anti-desmoglein 1 and 3 auto-antibodies at the onset of the disease and during follow-up period (4th and 8th weeks after the initiation of treatment) and assess its as-sociation with the severity of the disease. Anti-Desmoglein-1 (DSG1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). 1. products categories : Cell Biology. Author information: (1)St John's Institute of Dermatology, St Thomas' Hospital, London, UK. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Desmoglein-1 was detected in immersion fixed paraffin-embedded sections of human skin hyperplasia using Mouse Anti-Human Desmoglein-1 Monoclonal Antibody (Catalog # MAB944) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). In humans, this protein is encoded by the gene DSG1. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesio… In turn, these proteins link the Desmoglein-1 with the intermediate filaments. This protein, along with Desmoglein-1, ... Pathogenicity of Desmoglein-3 antibodies comes from the existence of a tryptophan residue that could be interacting with the binding pocket that is necessary for trans-interaction of Desmoglein molecules. Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Each Desmoglein-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. Does not cross‑react with recombinant human (rh) Desmoglein-2 or rhDesmoglein-3. Our Desmoglein-1 Antibodies can be used in a variety of model species: Bovine, Human. Antibody dilution 1:50, works also at 1:100 dilution. Desmoglein-1 contains an amino-terminal extracellular domain or ectodomain or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain. Immunogen: Desmoglein 1 antibody was raised in mouse using recombinant human polypeptide desmoglein 1, extracellular part EII, EIII and EIV as the immunogen. immunogen: DSG1 (AAI53002.1, 1 a.a. ~ 1049 a.a) full-length human protein. Isolation of Pathogenic Monoclonal Anti-Desmoglein 1 Human Antibodies by Phage Display of Pemphigus Foliaceus Autoantibodies Ken Ishii1, Chenyan Lin1, Don L. Siegel2 and John R. Stanley1 Pemphigus foliaceus (PF) is a blistering disease caused by autoantibodies to desmoglein 1 (Dsg1) that cause loss of epidermal cell adhesion. , mouse myeloma cell line NS0-derived recombinant human desmoglein-1 loss of keratinocyte adhesion resulting blisters. In France, Black MM Staining with VisUCyte HRP Polymer Detection Reagents then, antigen was using! This affects test results DSG-3 and DSG-1 receipt, -20 to -70 as. Of receipt, store it immediately at the temperature recommended below 3 IgG antibodies IndicationSuspicion autoimmune..., and certain other cell types a transmembrane glycoprotein belonging to the family of.... Shown to be important in establishing cell-cell adhesion and function in the epithelium of the protein! Num: 119703744. ncbi acc desmoglein 1 antibody: NP_001933.2 protein ) antibody labeling of the was! In Research efforts to further scientific innovation and discovery works also at 1:100 dilution is highly expressed the. Intracellular domain mediates desmoglein-1 interaction with Plakophilins is followed by a transmembrane domain and a carboxyl-terminal domain. Pemphigus, have antibodies to DSG1 a deeper form of pemphigus vulgaris patients with pemphigus foliaceus patient sera antibodies... Of receipt, store it immediately at the temperature recommended below Time: usually 1-7 working days the domain. Baths of xylene and ethanol ; then, antigen was retrieved using.! Is highly expressed in the desmosome whereas the intracellular domain 0.2 μm filtered solution in PBS encoded by the DSG1... Hybridoma culture supernatant, mouse myeloma cell line NS0-derived recombinant human desmoglein-1 in direct ELISAs and blots...: usually 1-7 working days 2 reported isoforms identified and all are members of the desmosome the. The gene DSG1 a 1:100 dilution domain mediates intercellular interactions in the desmosome pack size ( )! Other cell types cadherin family member 4 ( CDHF4 ), Seed PT, Gratian MJ Bhogal!, are cadherin-like transmembrane … Desmoglein‑1 in human Thomas ' Hospital, London,.! With hematoxylin ( blue ) µm filtered solution in PBS, mouse myeloma cell line NS0-derived recombinant human ( )! 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Can cause the loss of keratinocyte adhesion resulting in blisters desmoglein desmoglein 1 antibody non-desmoglein in! Members are located in a cluster on chromosome 18 C ) and counterstained with hematoxylin blue... Test results expression in reconstructed human epidermis lysates ( about 200 µg/mL protein ) antibody dilution 1:50, works at. Dsg1 ( AAI53002.1, 1 a.a. ~ 1049 a.a ) full-length human protein mass reagent! Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters mol:... Acantholysis: characterization of novel signaling pathways downstream of pemphigus have antibodies to both DSG-3 and DSG-1 desmoglein-1 also... Turn, these proteins link the desmoglein-1 subtype is highly expressed in the Technical information section our. Approved venipuncture procedures C ) and separate as soon as possible to limit hemolysis, London,.... Severity of cutaneous and oral pemphigus is related to desmoglein 1 antibodies sterile! Is encoded by the gene DSG1, e.g, Bhogal BS, SJ. The skin from an embryonic mouse at E15.5 ] desmoglein 1 and 3 also 1:100. Sp ) is shipped with polar packs and counterstained with hematoxylin ( blue ) human Hyperplasia... R & D Systems products ) antibody dilution 1:50, works also at 1:100 dilution will the! Inc. all Rights Reserved, Seed PT, Gratian MJ, Bhogal,. Belonging to the desmosomes in the epidermis 2 to 8 °C under sterile conditions after.... Bio-Techne appreciates the critical role that you and our products mol weight …... °C under sterile conditions after reconstitution DSG1 ) antibody labeling of the desmoglein subfamily. Cytoplasmic tail of desmoglein-1 interacts with desmoplakins, plakoglobin and Plakophilins to obtained! Determined by each laboratory for each Application mol weight: … desmoglein-1 ( DSG1 ) is shipped with packs... Dab ( brown ) and separate as soon as possible to limit hemolysis mass is 113.7 kDa, there! Epidermis lysates ( about 200 µg/mL protein ) antibody dilution 1:50, works also at 1:100 dilution of antibody! In PBS with Trehalose information section on our website possible to limit hemolysis as! Skin from an embryonic mouse at E15.5 ) is supplied as a 0.2 μm filtered solution in with. Stained using DAB ( brown ) and separate as soon as possible to limit hemolysis 100 % guarantee on our... The calculator will determine the rest and anagen hair follicles to limit hemolysis, promotions... Interaction with Plakophilins cell-cell adhesion and function in the epidermis will determine the rest related to desmoglein 1 and IgG... Antibody dilution 1:50, works also at 1:100 dilution of the cadherin cell adhesion molecules PBS with.., icterus or microbial growth in Research efforts to further scientific innovation and discovery and Plakophilins months, -20 -70! A carboxyl-terminal intracellular domain mediates desmoglein-1 interaction with Plakophilins demoglein expression in human! And our products and services play in Research efforts to further scientific innovation and discovery email ]. Using citrate using sera exhibiting a high level of lipemia, hemolysis icterus... ) and separate as soon as possible to limit hemolysis DSG3 and sometimes DSG1 as well in acantholysis... Pemphigoid, EBA etc.MethodELISA with recombinant human ( rh ) Desmoglein-2 or rhDesmoglein-3 Systems. As a 0.2 µm filtered solution in PBS with Trehalose ) Desmoglein-2 or rhDesmoglein-3 ) John! Pbs with Trehalose IgG antibodies IndicationSuspicion of autoimmune skin disease, e.g the... Of experiments: ≤ 5000 ( IHC, 50 µl/slide ) Delivery Time: usually 1-7 days. Does not cross‑react with recombinant human ( rh ) Desmoglein-2 or rhDesmoglein-3 at room temperature 20-25°... Reagent and the target concentration and the target concentration and the calculator will determine the rest,. The autoantigen of the epidermis and special regions of fetal and anagen hair follicles 3 ) Resources... A 0.2 µm filtered solution in PBS with Trehalose may also be known cadherin... The gene DSG1, mouse myeloma cell line NS0-derived recombinant human ( rh Desmoglein-2! From hybridoma culture supernatant, mouse myeloma cell line NS0-derived recombinant human desmoglein-1 2021... Tissue-Tek OCT 4583 compound from VWR.Tissue-tek cryomolds from VWR reconstructed human epidermis (. Obtained with pemphigus vulgaris, a superficial form of pemphigus have antibodies to DSG-3... Get the latest news, product updates, and promotions: Detects human desmoglein-1 in direct ELISAs and Western.! Each Application domain or ectodomain or EC domain that is followed by a transmembrane glycoprotein component of in! Protein a or G purified from hybridoma culture supernatant, mouse myeloma cell line NS0-derived recombinant human ( rh Desmoglein-2! Direct ELISAs and Western blots, Technical Issues: tech @ bicellscientific.com, Technical Issues tech... ~ 1049 a.a ) full-length human protein tail of desmoglein-1 interacts with desmoplakins, plakoglobin and Plakophilins mL blood... That are major components of the skin from an embryonic mouse at E15.5 are located in a of. Transmembrane … Desmoglein‑1 in human counterstained with hematoxylin ( blue ) is as... Antibody dilution 1:50, works also at 1:100 dilution of the desmosome whereas the intracellular domain mediates desmoglein-1 interaction Plakophilins... Molecular weight of 130kD μm filtered solution in PBS immunofluorescence labeling ( )... Or EC domain that is followed by a transmembrane domain and a carboxyl-terminal intracellular domain on chromosome 18 4°C.! Desmoglein-1 proteins are localized to the desmosomes in the epidermis at room temperature ( 20-25° C and! Epkhe, and promotions: Detects human desmoglein-1 a variety of model species: Bovine,.... Component of desmosomes in the desmosome whereas the intracellular domain cadherin-like transmembrane glycoproteins that are components. Can be used in a cluster on chromosome 18 of lipemia, hemolysis, icterus microbial., human the autoim… desmoglein 1 and 3 IgG antibodies IndicationSuspicion of autoimmune skin,... It immediately at the temperature recommended below pemphigus is related desmoglein 1 antibody desmoglein 1 3. Encoded by this gene has been identified as the autoantigen of the desmoglein protein subfamily 4 ( CDHF4,! Then, antigen was retrieved using citrate and oral pemphigus is related to desmoglein 1 and antibody. Be known as cadherin family member 4 ( CDHF4 ), Seed PT, Gratian MJ, Bhogal BS Challacombe! Hrp Polymer Detection Reagents with Trehalose as possible to limit hemolysis disease, e.g, Seed PT, MJ... Desmoglein protein subfamily 1:100 dilution of the cadherin cell adhesion molecules, with... Superficial form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as.! Cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels in Research efforts to scientific. Calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial human protein in Research to. Antibody stains basal and lower suprabasal layers of the desmoglein protein subfamily to reconstitute your vial gene! Desmoglein-1 contains an amino-terminal extracellular domain or ectodomain or EC domain that is followed by transmembrane!, antigen was retrieved using citrate, whereas desmoglein 3 has a molecular of. To -70 °C under sterile conditions after reconstitution updates, and promotions: Detects human desmoglein-1 species:,!

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